The Food To Be Absorbed Digestive Enzymes Biology Essay

The digestive system is made up of the digestive tract-a series of hollow variety meats joined in a long, writhing tubing from the oral cavity to the anus-and other variety meats that help the organic structure interrupt down and absorb nutrient.In order for the nutrient to be absorbed digestive enzymes are needed. Digestive enzymes are enzymes that interrupt down polymeric supermolecules into their smaller edifice blocks, in order to ease their soaking up by the organic structure.

Digestive enzymes are diverse and are found in the spit secreted by the salivary secretory organs, in the tummy secreted by cells run alonging the tummy, in the pancreatic juice secreted by pancreatic duct gland cells, and in the enteric ( little and big ) secernments, or as portion of the liner of the GI piece of land.Diastase was the really first enzyme discovered. It is the natural signifier of amylase.

This enzyme helps interrupt down saccharides and turn them into sugar, which makes them easier to digest. The enzyme can besides be found and extracted from beginnings such as milk, spit and other workss. Diastase helps in digestion of dietetic amylum, fats and lipoids, proteins etc.Starch is merely a carbohydrate consisting of big figure of glucose molecules.

This as mentioned before can be digested by the enzyme diastase.Peoples who have lack of this enzyme might develop jobs digesting certain nutrients. Hence conditions like acid reflux and such occur. In such instances nutrients like honey and milk can be consumed as it of course contains this enzyme or they might hold to take the enzymes addendums.

Purpose:

To analyze the rate at which amylum ( 10 % ) digests under different concentrations f diastase enzyme ( 0 to 5 % ) .

Hypothesis –

The enzyme diastase digests starch into malt sugar. My hypothesis for this experiment is that as the concentration of diastase enzyme increases the clip taken for the amylum to be digested lessenings.

Variables:

Independent variable –

Concentration of enzyme ( % )

0 % – 10ml of H2O ( diastase non added )1 % – 10ml of H2O and 0.10g of diastase2 % – 10ml of H2O and 0.20g of diastase3 % – 10ml of H2O and 0.

30g of diastase4 % – 10ml of H2O and 0.40g of diastase5 % – 10ml of H2O and 0.50g of diastaseFigure 1 – 0.50g of diastase for 5 % diastase solution.Degree centigrades: UserssanjayAppDataLocalMicrosoftWindowsTemporary Internet FilesContent.WordPhoto-0072.jpg

Dependant variable –

Time taken for amylum to digest ( seconds )

Control variable –

Glass wares used,Sum of amylum in starch solution ( 2ml of amylum ) ,Sum if I ( 2ml ) andSum of amylase ( 2ml )

Materials required:

DiastaseTest tubing5 Measuring cylinders3 DroppersElectric balanceIodine milliliterWater milliliterStarch 10gHot home baseMagnetic scaremongerMagnetGlass jars 3Distilled H2OStop ticker

Preparation of Diastase solution:

0 % – 10ml of H2O ( diastase non added )1 % – 10ml of H2O and 0.

10g of diastase2 % – 10ml of H2O and 0.20g of diastase3 % – 10ml of H2O and 0.30g of diastase4 % – 10ml of H2O and 0.40g of diastase5 % – 10ml of H2O and 0.50g of diastase

Method:

I measured 100 milliliter of H2O and 10 g of amylum.Figure 2 – 10g of amylumDegree centigrades: UserssanjayAppDataLocalMicrosoftWindowsTemporary Internet FilesContent.WordPhoto-0071.jpgI prepared a solution of 10 % amylum concentration by fade outing the amylum in hot H2O and magnetic scaremonger.

I so prepared the enzyme solution by taking different concentration of diastase ( as mentioned in the stuffs used ) and fade outing it in 10ml of distilled H2O.I did so for each concentration – 1 % , 2 % , 3 % , 4 % , 5 %Figure 3 -Prepared diastase enzyme solution in %Degree centigrades: UserssanjayAppDataLocalMicrosoftWindowsTemporary Internet FilesContent.WordPhoto-0073.

jpgFigure 4 – 2ml amylum + 2ml IDegree centigrades: UserssanjayAppDataLocalMicrosoftWindowsTemporary Internet FilesContent.WordPhoto-0076.jpgI so started the tests by blending 2 milliliter of I ( iodine so that the clip taken to fade out the amylum can be noted ) to 2 milliliter of amylum solution after which 2ml of diastase solution is added giving the solution a bluish colour as amylum is present.

Immediately after I added the diastase solution ( get downing with 0 % ) I started the halt ticker.The bluish colour given by I will vanish every bit shortly as the full amylum is digested. Once the bluish colour is replaced by white, I stop the ticker and enter the clip taken for the colour alteration ( the amylum to digest ) .I do this for all concentrations of enzyme up to 5 % concentration.And this experiment is repeated 5 times implying 5 tests.

Consequence:

Table 1 -Concentration of enzyme in %+ 0.05 %Change recorded over clip in seconds+ 2.00 secondsTrial 1Trial 2Trial 3Trial 4Trial 5AverageSouth dakota

+

0 %No alteration over clip1 %140.

00154.04160.70159.24179.20158.6414.10608662 %38.4744.

3242.7047.0743.2343.163.123 %22.2328.7132.

5327.2230.3328.203.

884 %16.5621.5521.9628.2128.5123.

365.045 %11.6111.8313.

148.8712.9111.671.

70Graph 1 – Averages of clip taken to digest the amylum in different concentrations of enzyme.

Discussion:

The solution with 0 % diastase concentration remains bluish as the amylum is non digested since no enzyme is present to respond on it. Hence I have n’t included this reading in graph 2 which shows the norm.

The solution with 1 % diastase concentration takes around 158.64 seconds on an norm for the colour to alter from bluish to white ( for the amylum to be digested ) .The solution with 2 % diastase concentration takes around 43.16 seconds on an norm for the colour to alter from bluish to white ( for the amylum to be digested ) .

The solution with 3 % diastase concentration takes around 28.20 seconds on an norm for the colour to alter from bluish to white ( for the amylum to be digested ) .The solution with 4 % diastase concentration takes around 23.36 seconds on an norm for the colour to alter from bluish to white ( for the amylum to be digested ) .The solution with 5 % diastase concentration takes around 11.67 seconds on an norm for the colour to alter from bluish to white ( for the amylum to be digested ) .

I noticed, as the diastase concentration additions, the clip taken to digest the amylum decreases. These both are inversely relative. It is obvious though because, more the enzyme faster the reaction takes topographic point hence the amylum being digested and alteration in colour from bluish to white faster.The 2 % and 3 % enzyme reading did non hold much difference as seen in graph 2. A important difference is seen though between 1 % and 2 % enzyme ‘s reading.

Decision:

Restrictions –

Since I have taken the readings manually by a halt ticker, it widens the range for errors. Had I found another manner to mensurate the clip it takes for the amylum to be digested by varied diastase concentrations, it would hold minimized any range for errors.Besides the enzymes solution used for all the tests was made and used on the same twenty-four hours but it was done over the period of a few hours which might hold affected the quality of the enzyme solution.Since non much difference is seen in the readings of 2 % and 3 % there is a opportunity for some mistake as when I notice the difference between the readings of 1 % and 2 % it shows the appropriate sum of difference in the clip taken for the amylum to fade out.