Probiotics and Biotechnology Essay
Probiotics and Biotechnology
Bacterias are present in the intestine of every 1. Some of these are harmful while others are good for the wellness of host. Normally the per centum of good bacteriums is more but due to antibiotic intervention and other emphasis conditions the balance displacement towards the harmful bacteriums.
Such a status is called Dysbiosis and needs the intervention of Probiotics.Probiotics are “live micro-organisms which when administered in equal sums confer a wellness benefit on the host ” . Examples are Lactic acid bacteriums ( LAB ) , Bifidiobacteria, barm and B sp. The nutrient that is good for the growing of Probiotics is called Prebiotics is largely oligosaccharides. Probiotics and Prebiotics are combinely called synbiotics.Metchnikoff was the first 1 who observes that it is possible to modify the intestine vegetation. He observed that proteolytic bacteriums like clostridium, produce toxic substances that cause aging but lactic acid bacteriums can suppress the growing of proteolytic bacteriums.
Henery Tissure insulate theBifidiobacterium bifidium from brest fed babies and he urge these for diarrhial septic kids. These protect by displacing proteolytic bacteriums. Kollath use the term Probiotics for the microbially derived factors that stimulate growing.Probiotics are the good micro-organism that are good to the human being through certain facets. Lactic Acid bacterium aid in pull offing lactose intolerance. These bacteriums convert lactose into digestible lactic acid. Some of the other benefits of Probiotics are as follow:* Lowering the cholesterin degree by interrupt down the gall in the intestine.
* Lowering the blood force per unit area by the production of ACE-Inhibitor like peptides.* Protect from Infections by competitory suppression and bettering immune system of the host.* Lactobacillus bulgaricus shows anti carcinogenic activity by diminishing the activity of ?-glucuronidase.* Block the adhesion site for the pathogens.* Probiotics provide the counter enviornment for the pathogens.
A good probiotic should hold the undermentioned belongingss.
Invitro attachment to the epithelial cells and anti-microbial activity. Should hold resistant to bile, HCl and pancreatic juice.
Immune transition and GIT colonisation. Probiotics are available in the signifier of dairy merchandises, nutrient, tablets and sachets in the market, with pescribed composing of probiotic micro-organisms.Probiotics Biotechnology helps to get the better of the different jobs faced during their preparation into useable signifier.
Like, emphasis during preparation, accommodation in new environment and to vie with bing bacteriums in the organic structure. The field of biotechnology that trades with the pathogens alterations is called Patho-Biotechnology. First end of the biotechnology is to better external emphasis tolerance, like tempreture and H2O. Naturally micro-organism do this by accumalating compatible solute e.g betaine and trehalose. These solutes stabilized the proteins and cut down H2O loss. The chief end of the biotecnology is to bring forth compatible solute accumalating strains, Listeria monocytogens has three natural uptake systems. BetL system has been selected which is encoded by one cistron, it is betaine transporter.
This cistron is cloned into Lactobacillus salivarius and Positive strains shows immune towards salts and more betaine consumption. The 2nd end of the biotechnology is to better the colonisation in the Gastrointestinal piece of land. This is acieved by cloning BetL cistron into B. breve and Trehalose cistron from E.
coli into L.lactis. The colned strains shows tolerence to gastric juice, gall and enteric colonisation. Third end of probiotics biotechnology is to bring forth the strains with increased pathogen competency. It has been observed that pathogens used oligosaccharides express on host cell as a receptors to adhere themselves. Through biotechnology attack receptor-mimic construction express strains are produce. The pathogens bind with these constructions and do them harmless for host every bit good as probiotic strains. E.
coli tha express chimeric lipopolysaccharide which is shiga toxin ( Stx ) receptor, merely 1mg can neutralized 100ug Stx.Inaddition to the alteration of bing system, artifically engineer improved systems are besides used. This technique is called Direct Evolution Approach.
In this attack mutant E.coli strain XL1-Red, which lack DNA mending system. It has mutant rate 5000 times higher than the normal strain, mutant may consequences into improved quality strains which are selected.
Like hyperproline bring forthing E.coli is osmotolerant, bring forth by the mutant in the first enzyme which consequences into the loss of provender back suppression and proline overrun.Normally probiotics has no side effects but may do infection in immunosuppressive people. If probiotics are chemically contaminated may be harmfull. So, probiotics are existent gift from God and we have to care for them.