Dna Gel Electrophorosis Essay Research Paper Introduction
Dna Gel Electrophorosis Essay, Research PaperIntroduction:Deoxyribonucleic acid, Deoxyribonucleic acid, is a dual stranded, coiling nucleicacid molecule which determines familial construction of a protein. The? stairss? are made of bases: A, G, C, and T. Thesides are sugar and phosphate molecules. Restriction enzymes areenzymes that cut DNA at limitation sites, go forthing fragments blunt orsticky. The limitation fragments are separated utilizing a technique calledgel cataphoresis.Deoxyribonucleic acid has a negative charge so when an electrical charge isapplied it makes DNA move to the positive side. Deoxyribonucleic acid is placed inagarose gel.
Smaller fragments move faster. The intent of this lab is toseparate Deoxyribonucleic acid fragments utilizing gel cataphoresis. Hind III cuts AAGCTTbetween the two irst A? s. EcoRI cuts at GAATTC between the G and theA. Hind III and EcoRI both make gluey terminals.Consequences:Our consequences for this lab were EcoRI separated into five fragments.Hind III separated into four fragments. The control merely had one fragment.
( See chart A and figure 1-1 for distances )Discussion:The intent of this lab was to see how gel cataphoresisoffprints DNA fragments. We used Hind III, EcoRI, and a controlledenzyme. Some fragments were difficult to see because of smearing. Thesewere the bigger fragments. Loading the Deoxyribonucleic acid was hard and if youweren? T careful you could tear the Wellss which ruined the lab. We,fortuitously, did non run into this job.
Abstraction:The intent of this lab is to divide DNA fragments with gelcataphoresis utilizing EcoRI and Hind III. Restriction enzymes are used tointerrupt up the Deoxyribonucleic acid, so negatively charged DNA is placed in a gelprojecting tray. Then it is placed into an electrophoresis chamber. Anelectrical field is placed across the agarose gel which forces thefragments to travel down the gel. The sum of lines show how manyfragments there is in the Deoxyribonucleic acid. We had five fragments for EcoRI and sixfor Hind III.
The no enzyme had merely one fragment.Procedures:We sealed the terminals of a gel projecting tray with dissembling tape andinserted a comb into the slots. The tray was filled about 6mm high withagarose gel. It covered half the tallness of the comb. We waited 10sproceedingss for the gel to solidify. Then we placed the tray in a gel box andmade sure that the comb was at a negative ( black ) terminal. The box wasfilled with tris-borate-EDTA buffer so it covered the full surface of thegel. The combs were removed without rending the Wellss.
The micro pipetwas used to lade the lambda EcoRI, lambda Hind III, and lambda merelyinto the Wellss. We dipped the pipet trough the surface of the buffer overthe Wellss and expelled the contents. The top of the cataphoresischamber was closed and electrical leads were connected. The dye wasobserved as it moved shortly after the power supply was turned on. Thepower supply was turned off after the sets migrated near the terminal ofthe gel and the top of the cataphoresis chamber was removed.
Weremoved the gel from the gel projecting tray and examined it under a visible radiationbox and compared it to the ideal gel ( figure1-2 ) .:Restriction Enzymes: Cleavage of DNA labUniversity of Illinois. ( 1999 ) . Experiment 2Gel Electrophoresis of DNA.
InMolecular Biology Cyberlab, online:Hypertext transfer protocol: //www.life.uluc.edu/molbio/geldigest/electro.html