There is immense one-year loss of both agricultural land and harvests due to different grounds like building, erosion of land, harvest diseases, plague onslaughts, desertification, etc. United Nations Environment Program has done a recent survey that showed that the nutrient concatenation managed by worlds is another ground for the cachexia of nutrient produced on a planetary degree. An one-year two percent increase in the sum of nutrient produced is required to run into the basic demands of human existences in footings of hungriness.
Harmonizing to Abbas ( 1989 ) , Asiatic states like Pakistan and India are sing the worst status due to greater figure of dwellers. Harmonizing to APO ( 2002 ) , merely half the figure of harvests as compared to developed states is produced in Pakistan. Sujatha et al. , ( ) says that the develpoing states need immense scientific support for the production of more harvests as a consequence of increasing population. Resultantly, there is figure in of import pulsations harmonizing to different research workers ( FAO, 1983 ; ICRISAT, 1991 ) . an increase demand of the latest agricultural direction and newest engineerings in order to achieve the needed ends.
Mehra ( 1993 ) has found in his survey that both developed and developing states have formulated new engineerings so as to heighten the quality of the harvests. In this respect, micropropagation is a really promising technique. Scowcraft and Ryan ( 1985 ) has reported that tissue civilization techniques have been adapted for the betterments of those workss that have immense pecuniary benefits. Harmonizing to Mehra ( 1993 ) , most new techniques in this respect are the in vitro regeneration system, development of noval loanblends through somantic hybridisation and formation of transgenic workss.
Different research workers ( Flick et al. , 1983 ; Barna and Wakhlu, 1993 ) have shown that although different workss have increased their quality via cellular technique but still there are workss like the leguminous plants that do non react to the in vitro regeneration system and therefore have non been improved. For these workss other new techniques need to be developed in the development states. In this respect, in vitro regeneration and clonal extension demand to be improved first of all as these are really of import techniques.
These leguminous plant workss need that much attending because they are economically really of import as nutrient stuff and because they fix nitrogen nowadays in the ambiance and without them this procedure can non be completed. Grain legumes, another name for which is pulse harvest, is a critical member of household Fabaceae, which in bend occupies 2nd rank in the line of blooming workss in nature.
A Chickpea is the most of import leguminous plant as it is on 3rd figure in the line of pulsations ( FAO, 1983 ; ICRISAT, 1991 ) . Harmonizing to Anonymous ( 2004 ) , an country of 982.3 thousand hour angle was used for the cultivation of garbanzo in 2003-04 and 611.1 thousand dozenss was the production with addition of 622 kg ha-1. But still chickpea output is really less in Pakistan. There are both aboitic and biotic factors responsible for this decrease in addition like Ascochyta blight, Botrytis grey cast, dry root putrefaction, collar putrefaction, Fusarium wilt, cod bore bit, dearth, salt, and low temperature. JAYANAN et al. , ( 2003 ) reported that betterments in confrontation with diseases and plagues can increase the output of this of import beginning of proteins.
In vitro technique is really of import because it has the ability, unlike the late available engineerings that are non plenty, to get the better of the issues of increasing universe population and diminishing land handiness. Direct organogenesis has been employed by different research workers for the regeneration of Cicer arietinum ( Kartha et al. 1981 ; Islam et Al. 1995 ; Barna, Wakhlu 1995 ; Anju, Chawla 2005 ) .
Tissue Culture Studies in Legumes
Purseglove ( 1968 ) reported the presence of 690 genera and 18,000 species in household Leguminosae. After the household Gramineae, it comes on 2nd figure and out of them merely 18-20 species are cultivated ( Aykroyd & A ; Doughty, 1964 ) . Harmonizing to National Academy of Sciences ( 1979 ) seeds of this household are critical nutrient supply and are 2nd merely to cereals and this household is tantamount to high saccharide nutrient like cereals and root tubers. This is of critical importance as developing states are sing decrease in protein degrees ( Mayer, 1976 ) .
Even though regeneration is hard for leguminous plants, but Nickell ( 1956 ) developed in vitro regeneration for this household. Different research workers so developed this techniques for different members of this household like mungbean ( Gulati and Jaiwal 1990, 1994 ) , peanut ( McKently et al. 1989 ) , Phaseolus vulgaris ( Malik and Saxena 1992a ) , pea, garbanzo, lentil ( Malik and Saxena 1992b ) and pigeonpea ( Prakash et al. 1994 ) . Different leguminous plants were regenerated like Hinchee et al. , 1988 ) , lentil ( Mahmoudian et al. , 2002 ) , blackgram ( Saini et al. , 2003 ) , and pigeon pea ( Thu et al. , 2003 ) utilizing their cotyledonary nodes as they showed the best response. Even though in vitro regeneration was used but rate of regeneration was rather low in some researches ( Flick et al. , 1983 ; Kysely et al. , 1987 ; Prakash et al. , 1994 ) . Malik and Saxena ( 1992b ) worked on P. vulgaris and found high sum of Benzyladenine to be really utile for shoot regeneration. Initiation of multiple shoots was besides found to be effected by this substance in mungbean ( Gulati and Jaiwal 1994 ) and pigeonpea ( Prakash et al. 1994 ) .
The Chickpea ( Cicer arietinum ) Plant
Van der Maesen ( 1972 ) reported that garbanzo ( besides called chickpea, gm and Bengal gm ) is grown in half dry conditions and its pollenation is besides self-induced. As for its name, “ Cicer ” is fundamentally a Latin word that has its beginning in the Greek word “ Krios ” from Roman household “ Cicero ” . Harmonizing to Westphal ( 1974 ) since the seed resembles the form of a random-access memory, so its species name i.e. Arietinum is derived from Latin word “ Rams ” . It comes 3rd on the line of pulse harvests in the universe ( FAO, 1983 ; ICRISAT, 1991 ) . It contains saccharides ( 48.2-67.6 % ) , amylum ( 41-50 % ) , proteins ( 12.4-31.5 % ) , fat ( 6 % ) other critical minerals ( Ignacimuthu and Prakash, 2006 ) .
Vegetarians give immense importance to this harvest as a rich protein supply and extremely adult harvest. Value of its straw for animate beings is tantamount to other straws. Alternate agribusiness that depends on N obtained from arrested development besides values this harvest a batch as it has the capableness to obtain more than 70 % nitrogen through the procedure of symbiotic dinitrogen arrested development. Kabuli-types and desi-types are the two types of agriculturists of this harvest.
Tissue Culture Studies in Chickpea
Bodily embryogenesis and embryo civilization
Different research workers ( Rao and Chopra, 1987, 1989 ; Riazuddine T al. , 1988 ; Rao, 1990, 1991 ; Dineshkumare t al. , 1994 ; Sonia et al. , 2002 ) have provided different procedures of regeneration including bodily embryogenesis and shoot organogenesis. Mature ( Rao and Chopra, 1989 ) and immature cusps ( Barna and Wakhlu, 1993 ; Kumar et al. , 1994 ) , immature seed leafs ( Eapen and George 1994, Hita et al. , 1997 ) mature ( Suhasini et al. , 1994 ) and immature embryo axes ( Sagare et al. , 1993 ) , or cell suspension civilizations ( Prakash et al. , 1994 ) are the sites that have ben used for bodily embryogenesis. Krishnamurthy et al. , ( 2000 ) used Kantrex and phosphinotricin for shoot regeneration of chickpea embryo axes, but these were non detailed estimations.
Different samples of chick pea like immature cotyledonary sections, immature embryo axes and mature embryo axes of cultivar PG12 were cultured and tissues were studied for bodily embryo induction and development. Bodily embryos were cut into 10 I?m pieces, treated with hematoxylin-eosin and eventually were seen through a microscope. Sagare et al. , ( 1995 ) reported that for immature seed leafs and immature embryo axes, bodily embryos base on balls through a superseding callosity stage for their formation, while path is direct for mature embryo axes and for immature cusps it can both be direct or indirect.
Shoot buds can besides be regenerated via either direct ( Shri and Davis, 1992 ; Kar et al. , 1996 ; Sharma and Amla, 1998 ; Subhadra et al. , 1998 ) or indirect waies ( with an intermediate callosity formation stage ) ( Khan and Ghosh, 1984 ; Prakash et al. , 1992 ; Barna and Wakhlu, 1994 ) . But merely cotyledonary nodes and shoot vertexs have yet been used for chick pea regeneration ( Sonia et al. , 2002 ) .
Jayanand et al. , ( 2003 ) , utilizing cotyledonary nodes of in vitro germinated seedlings of biddy pea, obtained a sample from alar meristems and gave a really adept and high rated manner to renew shoots of this works. Furthermore, Rekha and Thiruvengadam ( 2009 ) used the cotyledonary nodes and alar buds of this works and utilizing them gave a high frequence and replicable method of regeneration of the plantlets every bit good as in vitro shoot initiation.
Axillary shoot proliferation ( ASP )
Axillary shoot proliferation can be used for micropropagation of garbanzo ( 1, 5, 6 ) . Even though a true-to-type method, still the velocity of this method is rather slow ( 11 ) . Axillary shoot proliferation and modified individual node civilization were the two methods used for micropropagation of this works. Murashige and Skoog ( MS ) medium enriched with 1-10 pM N6-benzyladenine and 0.01 pM a-naphthaleneacetic acid gave the maximal coveted consequences in signifier of propagules. These were placed in the MS medium that has 1 autopsy 3-indolebutyric acid and B5 vitamins. Plants therefore produced were productive and their visual aspect was same to those workss grown from seeds. Barna and Wakhlu ( 1995 ) showed that with greater figure of workss produced per sample, MSNC is more successful regeneration method.
Using already present meristems is hopeful to take to a more successful and rapid regeneration method. Inspite of different available methods, regenerated shoots were really limited in respect to rooting. These methods were gene-specific and for most of the clip were non even replicable. Resultantly, shoot initiation and in vitro rooting demands more attending.
Affect of growing regulators in organogenesis and works regeneration
Different research workers used different stuffs for shoot regenaretion, expansion, and for organizing roots in ICCV-10 and Annigeri samples of biddy pea. These stuffs were phenylacetic acid ( PAA ) ( Ghanti et al. , 2009 ) every bit good as of course happening auxins present in many lower and higher works species ( Abe et al. 1974, Schneider and Wightman 1986 ) . No old surveies have yet been found in which PPA was used and direct regeneration occurred. In all the instances, an intermediate callosity phase was besides observed. Malik and Sexana ( 1992b ) reported about the importance of thidiazuron in multiple shoot initiation of garbanzo. Benzyladenine ( BA ) and its function in multiple shoot initiation have besides been examined. Type and familial construction of the seed sample every bit good as the sum of BA were the factors that affected the distinction of shoots. This shoot distinction was besides effected by way of the sample. Polisetty et al. , ( 1997 ) found that genotype is the factor that influences what the measure of BA should be for shoot/shoot bud regeneration. Another survey found that when benzyladenine is assorted with either indolacetic acid or with naphthaleneacetic acid, so the shoot regeneration from the hypocotyls samples is extremely influenced in a positive manner ( Islam et al. , 1994 ) . However, one drawback was that the sum of shoots obtained for each sample that was regenerated was rather low i.e. merely 6-8 shoots were obtained.